Human induced pluripotent stem cell-derived mesenchymal stem cells (hiPSC-MSCs) have emerged as a promising alternative for stem cell transplantation therapy. Exosomes derived from mesenchymal stem cells (MSC-Exos) can play important roles in repairing injured tissues.
Analysis of cellular mechanotransduction, the mechanism by which cells convert mechanical signals into biochemical responses, has focused on identification of critical mechanosensitive molecules and cellular components.
Mesenchymal stem cells (MSCs; also called mesenchymal stromal cells) have received much attention during the last two decades, at first because of their regeneration capacity and poor immunogenicity and, more recently, because of their proved immunomodulatory function.
Shock wave therapy (SWT) represents a clinically widely used angiogenic and thus regenerative approach for the treatment of ischaemic heart or limb disease. We hypothesize that SWT causes cellular cavitation without damaging the target cells, thus liberating cytoplasmic RNA that in turn activates TLR3.
Extracorporeal shock wave (SW) therapy has been studied in the transfection of naked nucleic acids into various cell lines through the process of sonoporation, a process that affects the permeation of cell membranes, which can be an effect of cavitation. In this study, siRNAs were efficiently transfected into primary cultured cells and mouse tumor tissue via SW treatment.
We show for the first time how the mechanical stimulus of SWT is translated into a biological response. SWT causes exosome release which have and highly angiogenic potential in vitro and in vivo. Exosomes released by SWT might develop a therapeutic treatment option for ischemic heart disease.
Cell-based therapies with autologous adipose tissue–derived cells have shown great potential in several clinical studies in the last decades. Although possible clinical applications of autologous adipose tissue– derived cells are manifold, they are limited by insufficient uniformity in cell identity and regenerative potency.
One of the mainstays of facial rejuvenation strategies is volume restoration, which can be achieved by autologous fat grafting. In our novel approach, we treated the adipose tissue harvest site with extracorporeal shock wave therapy (ESWT) in order to improve the quality of the regenerative cells.
Platelet-rich plasma (PRP) and extracorporeal shockwave therapy (ESWT) are both used for the treatment of soft tissue injuries in horses. Clinically, the question has been raised whether these two therapies could be used in combination. The hypothesis of this study was the application of ESWT to PRP would increase the release of platelet derived growth factors (PDGF) and transforming growth factors beta 1 (TGF) from platelets.
Results: ESWT enhances cell proliferation in vitro and wound healing in vivo. Conclusion: ESWT-induced ATP release and subsequent extracellular signal-regulated kinase (ERK) activation are prerequisites for enhanced cell proliferation and wound healing.
Our results demonstrate for the first time that hMSCs express functional P2X7 receptors and that shockwave treatment induces osteogenic differentiation by stimulating these P2X7 receptors by the release of cellular ATP.
The presented data describe fESW as a potential approach to manipulate hBMSC behavior for clinical applications. This approach appears particularly promising as it suggests that mechanical stress preconditions hBMSCs for improved therapeutic performance without any genetic manipulation or loss of differentiation potential.
Our investigation showed significant effects of the application of shock wave treatment on cell apoptosis with increasing severity of the shock wave conditions. Furthermore, an alignment of the f-actin fibers was demonstrated by phalloidin staining after shock wave application.
Principal Findings of the Current Study. A variety of animal models and some clinical trials have shown that EPC therapy is effective in improving limb ischemia. In the current study, we found that, compared with the CLI control group, the ratio of INBF was remarkably improved in the BMDEPC therapy group. The results of the current study, therefore, reinforce the findings of the previous studies
This study followed the hypothesis that enhanced Bone Morphogenetic Protein (BMP)-signaling in adult mesenchymal stem cells that are induced for tendon formation may overcome the tendon-inherent interference with bone formation and may thus allow the stem cell-dependent formation of tendon-bone interfaces.
Our study confirms that SW treatment can have a positive effect on endothelial cells proliferation and differentiation as well as on the number of primitive cardiomyocytes and smooth muscle cells in vitro.
Preconditioning of both nonischemic and chronic ischemic tissue with low-energy SW improves recruitment of circulating EPCs via enhanced expression of chemoattractant factors. Thus, SW-facilitated cell therapy may improve the efficacy of EPC treatment in patients with chronic ischemia.
Extracorporeal shock-wave (ESW) treatment has been shown to be effective in promoting the healing of fractures. We aimed to determine whether ESW could enhance the growth of bone-marrow osteoprogenitor cells.